MAZ antibody (mAb)

RRID: AB_2793398
Clone: 133
Aliases: PUR1, ZF87, Pur-1, SAF-1, SAF-2, SAF-3, Zif87, ZNF801
Catalog No: 39935 Format: 100 µg ¥5,610 Buy
Catalog No: 39936 Format: 10 µg ¥1,380 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
55 kDa
Reactivity:
Human

Applications

ChIP Validated Western Blot Validated Electrophoretic Mobility Shift Assay Validated

Application Notes

Applications Validated by Active Motif:
WB

This antibody is also available as an AbFlex® engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91205.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP
EMSA

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This MAZ antibody was raised against recombinant human MAZ protein.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
MAZ antibody (mAb) tested by Western Blot.

MAZ antibody (mAb) tested by Western Blot.
10 µg MCF7 cell nuclear extract was run on SDS-PAGE, and probed with 2 µg/ml AbFlex MAZ antibody.
MW: 55 kDa

Background

MAZ (Myc-associated zinc finger protein) functions as a transcription factor roles in both transcription initiation and termination. MAZ binds to two sites within the c-Myc promoter, ME1a1 and ME1a2. It also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors. MAZ also participates in skeletal muscle development through the activation of several skeletal muscle-specific genes. MAZ has also been reported to drive tumor-specific expression of PPAR-gamma in breast cancer cells.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with MAZ antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting