AbFlex® Histone H3K4me0 antibody (rAb, mouse)

RRID: AB_2793826
Catalog No: 91267 Format: 100 µg ¥4,240 Buy
Catalog No: 91268 Format: 10 µg ¥940 Buy

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Antibody Type:
Recombinant
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated Dot Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 0.1 – 1 µg/ml
Dot Blot: 0.5 - 1 µg/ml

Active Motif also offers AbFlex® Histone H3K4me0 antibody (rAb) expressed as full-length IgG with rabbit immunoglobulin heavy and light chains, Catalog No. 91317.

Immunogen

This antibody was raised against a peptide containing the N-terminus of human histone H3.

Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

View our Guide to Histone Modifications and Biological Function.

 
AbFlex<sup>®</sup> Histone H3K4me0 antibody (rAb) tested by Western blot.

AbFlex® Histone H3K4me0 antibody (rAb) tested by Western blot.
20 µg of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex Histone H3K4me0 antibody at 0.5 µg/ml.

AbFlex<sup>®</sup> Histone H3K4me0 antibody (rAb) tested by dot blot analysis.

AbFlex® Histone H3K4me0 antibody specificity is shown by Dot Blot.
Recombinant proteins were spotted onto PVDF as follows: Lane 1 - Histone H3 (C110A), Cat. No. 31207; Lane 2 - Histone H3K4me1 (EPL), Cat. No. 31287; Lane 3- Histone H3K4me2 (EPL), Cat. No. 31277; Lane 4 - Histone H3K4me3 (EPL), Cat. No. 31278. Top panel was probed with AbFlex Histone H3K4me0 antibody at 0.5 µg/ml. Bottom panel was probed with Histone H3 mAb (Clone 1B1-B2), Cat. No. 61475, at 1 µg/ml.

Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® H3K4me0 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes. This antibody recognizes the Histone H3, lysine 4, which is unmethylated.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting