Recombinant METTL16 protein

Catalog No: 81085 Format: 20 µg ¥3,920 Buy
Catalog No: 81785 Format: 1 mg ¥32,400 Buy

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

METTL16 (Methyltransferase-like protein 16), also known as U6 small nuclear RNA (adenine-(43)-N(6))- methyltransferase or METT10D (Methyltransferase 10 Domain-Containing Protein), is a RNA N6-methyltransferase that methylates adenosine residues of a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs). In contrast to the METTL3-METTL14 heterodimer, METTL16 is only able to methylate a limited number of RNAs: requires both a 5UACAGAGAA-3 nonamer sequence and a specific RNA structure. It also plays a key role in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. In presence of S-adenosyl-L-methionine, METTL16 binds the 3-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, leading to intron retention and preventing MAT2A mRNA splicing. While in S-adenosyl-L-methionine-limiting conditions, it binds the 3-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, leading to stimulate splicing of the MAT2A retained intron, and promoting expression of MAT2A.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

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Protein Details

Recombinant human METTL16 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_076991.3) with an N-terminal FLAG tag. The molecular weight of the protein is 64.9 kDa.

 
Recombinant METTL16 protein gel

Recombinant METTL16 protein gel
10% SDS-PAGE Coomassie staining
MW: 64.9 kDa
Purity: >95%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.