Cart
Products
NCAPH2 antibody (mAb)
| RRID: AB_2793733 |
| Clone: 5F2G4 |
| Aliases: CAPH2 |
| Catalog No: 61681 | Format: 100 µg | ¥5,500 | Buy |
| Catalog No: 61682 | Format: 10 µg | ¥1,380 | Buy |
Request a quote for a bulk order Request Quote
Product Review Submit a Review
Applications
Application Notes
Applications Validated by Active Motif:
ICC/IF: 1 - 2 µg/ml dilution
WB*: 0.5 - 2 µg/ml dilution
The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.
*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This antibody was raised against a recombinant protein to human NCAPH2.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
NCAPH2 antibody (mAb) (Clone 5F2G4) tested by immunofluorescence.
Formaldehyde fixed HeLa cell stained with the NCAPH2 antibody.
NCAPH2 antibody (mAb) (Clone 5F2G4) tested by Western blot.
NCAPH2 antibody detection by Western blot. The analysis was performed using 30 µg of HeLa nuclear extract and NCAPH2 antibody at a 1 µg/ml dilution.
Background
NCAPH2 (Non-SMC Condensin II Complex, Subunit H2) Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development. (By similarity)
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
