Histone H3K9me2S10ph / H3K27me2S28ph antibody (mAb)

RRID: AB_2793632
Clone: 51Ta2H12
Catalog No: 61429 Format: 100 µg Discontinued
Catalog No: 61430 Format: 10 µg Discontinued

Antibody Type:
Monoclonal
Isotype:
IgG1, k
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
17 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

Application Notes


This product has been discontinued


Published Applications:
IP
ICC/IF
WB
DB
ELISA
See references for more information. Individual optimization may be required.

Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acid residues 180-195 of human TAF10. The antibody recognizes H3 doubly modified at K9me2S10ph and/or K27me2S28ph.

Buffer

Purified IgG in 100mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.1 mM EDTA, 45 mM glycine, 0.05% sodium azide.

View our Guide to Histone Modifications and Biological Function.

Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.

Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Histone H3 tails can be dimethylated on lysine (Kme2) and also phosphorylated on the adjacent serine (Sph) residue. H3K9me2S10ph and/or H3K28S29ph have been observed to occur during mitosis and may provide a specific chromatin conformation during chromatin condensation for cell division.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting