Histone H3K9me0 antibody (mAb)
RRID: AB_2793620 |
Clone: 9B1-2G6 |
Catalog No: 61399 | Format: 100 µg | ¥5,500 | Buy |
Catalog No: 61400 | Format: 10 µg | ¥1,380 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 0.5 - 2 µg/ml dilution
Immunogen
This antibody was raised against a synthetic branched peptide corresponding to amino acids surrounding Lys9 of human Histone H3.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
Also available as an AbFlex™ engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91155.
View our Guide to Histone Modifications and Biological Function.
Background
Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Methylation of lysines occur as four states: unmethylated (me0), monomethyl (me1), dimethyl (me2) and trimethyl (me3). The me0 state of lysine is recognized as biologically relevant and a number of proteins containing PhD fingers, ADD and WD40 domains are known to associate with unmodified lysines.
Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3K9me0 antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting