OGT/O-GlcNAc transferase antibody (pAb)

RRID: AB_2793604
Aliases: HRNT1, O-GLCNAC
Catalog No: 61355 Format: 100 µl ¥5,610 Buy
Catalog No: 61356 Format: 10 µl ¥1,380 Buy

Request a quote for a bulk order Request Quote

Product Review Submit a Review

Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
120 kDa
Reactivity:
Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

Application Notes

Applications Validated by Active Motif:
ChIP: 5 µl per ChIP
ChIP-Seq & ChIP-chip: 5 µl each
WB: 1:500- 1:2,000 dilution

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-Seq
IHC(F), IF
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This antibody was raised against a recombinant protein corresponding to the N-terminal region of human OGT.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

 
OGT/O-GlcNAc transferase antibody (pAb) tested by ChIP-Seq.

OGT/O-GlcNAc transferase (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 3 million primary mouse T cells and 5 µl of OGT antibody. ChIP DNA was sequenced on the Illumina HiSeq and 14 million sequence tags were mapped to identify OGT binding sites. The image on the left shows many OGT binding sites within a 25 million bp region on mouse chromosome 8. The image on the right shows that binding sites are localized to gene promoters as expected based on the known interaction with the H3K4 methylating COMPASS complex.

OGT/O-GlcNAc transferase antibody (pAb) tested by Western blot.

OGT/O-GlcNAc transferase (pAb) tested by Western blot.
Detection of OGT/O-GlcNAc transferase by Western blot. The analysis was performed using 30 µg HeLa whole-cell extract and OGT/O-GlcNAc transferase (pAb) at a 1:500 dilution.

Background

OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to serine or threonine residues in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). OGT can glycosylate a large and diverse number of proteins including HCF1, AKT1, MLL5 and histone H2B. It can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Specifically OGT has been shown to interact directly with TET2 and TET3, enzymes that catalyze the oxidation of 5-methylcytosine on DNA. The TET/OGT interaction leads to GlcNAcylation of HCF1, a protein component of the COMPASS complex, which regulates H3K4 methylation and gene expression.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for OGT/O-GlcNAc transferase antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with OGT/O-GlcNAc transferase antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting