Sf3b3 antibody (mAb)

RRID: AB_2615003
Clone: 1YO-1B10
Aliases: Splicing Factor 3b Subunit 3, RSE1, SAP130, SF3b130, STAF130
Catalog No: 61345 Format: 100 µg Discontinued
Catalog No: 61346 Format: 10 µg Discontinued
Antibody Type:
Monoclonal
Isotype:
IgG1, k
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
135 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes


This product has been discontinued


Published Applications:
IP
WB
See references for more information. Individual optimization may be required.

Immunogen

This antibody was raised against a peptide corresponding to amino acid residue(s) 1057-1076 of human Sf3b3.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Background

Sf3b3 (splicing factor 3b, subunit 3, 130kDa) is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting