N-Myc antibody (pAb)

RRID: AB_2793543
Aliases: MYCN, NMYC, ODED, MODED, MYCNOT, bHLHe37
Catalog No: 61185 Format: 100 µl ¥5,610 Buy
Catalog No: 61186 Format: 10 µl ¥1,380 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
60 kDa
Reactivity:
Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

Application Notes

Applications Validated by Active Motif:
ChIP-Seq: 10 - 20 µl per ChIP
WB: 1:500 - 1:2,000 dilution

For optimal results in Western Blot, primary antibody incubations should be performed at 4°C. Individual optimization may be required.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-Seq

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This N-Myc antibody was raised against a peptide derived from the C-terminal region of human N-Myc.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

N-Myc antibody (pAb) tested by ChIP-Seq
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 200 µg of chromatin from human cell line chromatin and 20 µg N-Myc antibody. ChIP DNA was sequenced on the Illumina HiSeq and 9.2 million sequence tags were mapped to identify N-Myc binding sites on chromosome 3. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

N-Myc antibody (pAb) tested by Western blot.
Nuclear extract (20 µg) of Kelly cells probed with N-Myc antibody at a dilution of 1:1,000

Background

N-Myc (MYCN) is a member of the Myc family of proto-oncogenes. Myc family members play crucial roles in regulating cell proliferation, size and differentiation. N-Myc is required for proper development of the nervous system. Myc family proteins can induce malignant transformation inappropriately modulating gene transcription, leading to unchecked cell proliferation. Misexpression of N-Myc is often associated with pediatric neural cancers such as neuroblastoma, medulloblastoma and retinoblastoma.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with N-Myc antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting