SMC1 phospho Ser957 antibody (pAb)
RRID: AB_2793282 |
Aliases: SMC1A, SMCB, CDLS2, SB1.8, SMC1L1, DXS423E, SMC1alpha |
Catalog No: 39631 | Format: 200 µl | ¥5,500 | Buy |
Catalog No: 39632 | Format: 10 µl | ¥1,380 | Buy |
Request a quote for a bulk order Request Quote
Product Review Submit a Review
Applications
Application Notes
Applications Validated by Active Motif:
WB: 1:500 - 1:1,000 dilution
Immunogen
This SMC1 phospho Ser957 antibody was raised against a peptide containing phospho-serine 957 of human SMC1.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Background
The Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. SMC1 has a myosin-like ATPase domain that serves as a molecular motor to help organize chromatin and is part of the cohesin complex that facilitates chromosome cohesion during the cell cycle. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. SMC1 is also involved in DNA damage repair. Subsequent to double strand DNA breaks, SMC1 is phosphorylated by the ATM kinase. Phosphorylated SMC1 is crucial to the successful repair of DNA damage. Defects in SMC1 isoform A are the cause of Cornelia de Lange syndrome type 2, an inherited developmental disorder associated with malformations affecting multiple systems.
Positive Control
Nuclear extract of HeLa cells treated with ETO (100 µM for 2 hours) can be used as positive control for SMC1 phospho Ser957 antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting