STAT3 phospho Tyr705 antibody (pAb)

RRID: AB_2793272
Aliases: APRF, HIES
Catalog No: 39595 Format: 200 µl ¥5,500 Buy
Catalog No: 39596 Format: 10 µl ¥1,380 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
92 kDa
Reactivity:
Human

Applications

Western Blot Validated Dot Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 - 1:2,000 dilution

Immunogen

This STAT3 phospho Tyr705 antibody was raised against a peptide containing phosphorylated Tyr705 of human STAT3.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
STAT3 phospho Tyr705 antibody (pAb) tested by Western blot.

STAT3 phospho Tyr705 pAb tested by Western blot.
Hep G2 nuclear extract (15 µg per lane) was probed with STAT3 phospho Tyr705 polyclonal antibody (1:2,000).
    Lane 1: No treatment.
    Lane 2: cells stimulated with IL-6 (100 ng/ml for 15 minutes at 37°C).

STAT3 phospho Tyr705 antibody (pAb) tested by dot blot analysis.

STAT3 phospho Tyr705 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of STAT3 phospho Tyr705 pAb for STAT3 phospho Tyr705. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row.

Lane 1: Unmodified Ser727 STAT1 peptide. Lane 2: Phospho Ser727 STAT1 peptide. Lane 3: Unmodified Tyr689 STAT2 peptide. Lane 4: Phospho Tyr689 STAT2 peptide. Lane 5: Unmodified Ser727 STAT3 peptide. Lane 6: Phospho Ser727 STAT3 peptide. Lane 7: Unmodified Tyr705 STAT3 peptide. Lane 8: Phospho Tyr705 STAT3 peptide. Lane 9: Unmodified Ser726 STAT5A/Ser731 STAT5B peptide. Lane 10: Phospho Ser726 STAT5A/Ser731 STAT5B peptide. Lane 11: Unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide. Lane 12: Phospho Tyr694 STAT5A/Tyr699 STAT5B peptide.

Background

STAT3 (signal transducer and activator of transcription 3) is a member of the STAT family of transcription factors. STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNα, IL-10, IL-6, IL-11, IL-12, IL-2, EGF, among others.

Positive Control

Active Motif's Hep G2 nuclear extract (IL-6 stimulated, 100 ng/ml) (Catalog No. 36107) can be used as a positive control for STAT3 phospho Tyr705 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting