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Recombinant BRPF3 (576-701) protein
| Aliases: Bromodomain And PHD Finger Containing 3, KIAA1286 |
| Catalog No: 31487 | Format: 100 µg | ¥3,800 | Buy |
| Catalog No: 31787 | Format: 1 mg | ¥20,280 | Buy |
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Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
BRPF3 (Bromodomain and PHD finger containing 3) is a component of the MOZ/MORF histone acetyltransferase (HAT) complex. The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. Acetylated lysine residues can be recognized by bromodomain. The bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. There is less study on its function now.
Application Notes
Recombinant BRPF3 (576-701) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Protein Details
The peptide corresponding to amino acids 576-701 that contains the bromodomain sequences of BRPF3 (accession number NM_015695.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 19.7 kDa. The recombinant protein is >90% pure by SDS-PAGE.
Recombinant BRPF3 (576-701) protein gel.
BRPF3 (576-701) protein was run on an SDS-PAGE gel and stained with Coomassie blue.
Recombinant BRPF1 (576-701) HTRF activity assay.
3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (576-701) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-Flag antibody was used to detect reaction products.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
